Assessing mitochondrial dysfunction in cells

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چکیده

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Assessing mitochondrial dysfunction in cells

Assessing mitochondrial dysfunction requires definition of the dysfunction to be investigated. Usually, it is the ability of the mitochondria to make ATP appropriately in response to energy demands. Where other functions are of interest, tailored solutions are required. Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimenta...

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Assessing mitochondrial dysfunction in primary cardiomyocytes

This Application Note describes a method for profiling mitochondrial function in cells responding to stress. The mitochondrial profile generated in this way provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity. The results described here suggest that the development of cardiom...

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Assessing mitochondrial dysfunction in primary cardiomyocytes

This application note describes a method for profiling mitochondrial function in cells responding to stress. The mitochondrial profile generated in this way provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity. The results described here suggest that the development of cardiom...

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Assessing mitochondrial dysfunction in primary cardiomyocytes

This Application Note describes a method for pro ling mitochondrial function in cells responding to stress. The mitochondrial pro le generated in this way provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity. The results described here suggest that the development of cardiomyo...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2011

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20110162